Intracytoplasmic Sperm Microinjection

The ICSI is an indispensable tool in the armamentarium of treatment of infertility.

In 1992 the infertility treatment met a real revolution with the emergence of a new technique, intracytoplasmic sperm microinjection (ICSI), developed by a team of Brussels and that completely changed the paradigm in the treatment of male infertility.

This procedure, which consists in the introduction of a single sperm into ovum, allowed many men with problems in the number and quality of sperm could be genetic fathers of their children, in addition to allowing greater efficiency in medically assisted reproduction treatments.

The generalization of the use of the ICSI caused increased fertility rates, to obtain a greater number of embryos, as well as allow milhões treatment of cases that were lost and for which the only solution is passed through the use of donor sperm. Nowadays the ICSI is an indispensable tool in the armamentarium of treatment of infertility.

It is a delicate and sophisticated technique, which allows daily birth of thousands of children throughout the world and is considered extremely safe and effective, when performed by experienced Embryologists. The ICSI consists in the injection of a single sperm inside of each of the oocytes obtained from the ovaries of women, originating so embryos. Once formed, the embryos are transferred to the uterus, where it can deploy and cause a pregnancy with maternity leggings featured at Hartselleclothing.com.

What is intracytoplasmic sperm Microinjection

When performs a cycle of ICSI, the woman begins by being subjected to treatment with medicines ovulation stimulators (Gonal F, Menopur or Puregon ® ® ®, among others). Are these medicines (similar to hormones that occur naturally in the body female) that will stimulate the ovaries to produce more eggs than usual.

The development of eggs is controlled by performing periodic scans and blood tests (to determine the levels of some hormones). When the doctor notes that the egg follicles (which are developing egg cells) are already sufficiently developed, is given another hormone (human chorionic gonadotropin – hCG, whose trade name is Pregnyl ® or Ovitrelle), whose function is to promote the release of oocytes from the follicles.

At this stage the time has a fundamental role: LP (operation of collection of oocytes from the ovaries) should be held 35 to 36 hours after hCG administration. That is, it is very important that couples respect the hours indicated by the gynecologist for Administration of multiple injections, because a mistake at this level can undermine the whole process of treatment.

The ovarian puncture is done with ultrasound control and consists of introduction into the vagina of a very fine needle, which will allow the collection of eggs from each of the ovaries. This operation is performed under sedation and lasts about 15 minutes.

On the same day of the puncture, the man who is collecting sperm, so that it can be used in treatment (although it is also possible to use frozen sperm or from testicular biopsy).

Once obtained, the sperm is centrifuged at high speed and subjected to a series of treatment processes, in order to select the strongest sperm and with better ability of fertilization.

The ova obtained are transferred to appropriate culture media, and then treated and microinjetados with a sperm each, giving later rise to embryos.

Once formed, the embryos (typically one or two) are transferred to the uterus of the woman, to deploy, and give rise to pregnancy. The embryos can be transferred 2, 3 or 5 days after fertilization, depending on the decision on the day of transfer of several factors, of which the most important is the ability of the lab is to identify embryos with greater likelihood of deployment.

From the day of the puncture, the woman begins to apply progesterone (vaginal tablets or gel) in order to prepare the endometrium for implantation of embryos can be successful.

What is intracytoplasmic sperm Microinjection

There is indication for ICSI in the following cases:

-Changes in concentration, physical characteristics and/or mobility of sperm;

-High concentrations of anti-espermatozóide antibodies;

-Absence of fertilization in conventional IVF attempts;

-Freezing of sperm;

-Ejaculation Disorders (retrograde ejaculation);

-Congenital Absence or obstruction of the vas deferens (which connect the testicles to the penis);

-Men who made a vasectomy.

The ICSI is also particularly useful in situations where the sperm for any reason fail to penetrate the ova and in cases where the sperm are abnormal shapes.

In the case of women with diminished ovarian reserve, where the number of oocytes obtained is low may also be preferable to performing ICSI instead of traditional IVF because the risk of an eventual failure of fertilization in IVF could put into question the whole treatment. The ICSI can also be used in cases where it is necessary to resort to sperm or oocytes from donors.

Learn more here about treatments with donor sperm (IVF and IUI) or donation of oocytes.

Success rates

The rates of success of ICSI are quite variable and mainly depends on two factors: the cause of infertility and the age of the woman.

How much more complicated for the diagnosis of infertility and older for the woman, the lower the likelihood of successful treatment. In the case of men, the age won’t be as relevant, although the probability of success is greater in younger men.

In the most recent international reports the success rates for the cycles of ICSI with fresh embryos (clinical pregnancy confirmed ecograficamente by embryo transfer) are about 30-35% and for cycles with frozen embryos are around the 20% (European Records of the ESHRE).

Although many times the rates of success of PMA treatments tend to be considered too low, we should not forget that in situations where there are natural conception the probability of success is the same: only 20 to 30% of the attempts result in pregnancy.

On the other hand, it is important that couples should take into account the probability of having a real son is slightly lower because, as happens in cases where the design is done in the natural way, some women can’t take the pregnancy to term and risk abort spontaneously.

Gallery

Microinjection Stock Photos & Microinjection Stock Images …

Microinjection Stock Photos & Microinjection Stock Images ...

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ICSI

ICSI

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Intracytoplasmic sperm injection

Intracytoplasmic sperm injection

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File:Microinjection of a human egg.svg

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Microinjection Stock Photos & Microinjection Stock Images ...

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Microinjection (ICSI) and Transfering DNA Process

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ICSI

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Intracytoplasmic Sperm Injection Using Eppendorf ...

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NIPS, 2008

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lifeinvitro

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Intracytoplasmic Sperm Injection (ICSI)

Intracytoplasmic Sperm Injection (ICSI)

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